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Volume 85, Issue 5 p. 1663-1678
SOIL BIOLOGY & BIOCHEMISTRY

Diversity and biotechnological potential of rhizobia isolated from lima bean nodules collected at a semiarid region

Tainara Louzada Rodrigues

Tainara Louzada Rodrigues

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Contribution: Conceptualization, Data curation, ​Investigation, Methodology, Visualization, Writing - original draft, Writing - review & editing

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Elaine Martins da Costa

Elaine Martins da Costa

Univ. Federal do Piauí, Campus Professora Cinobelina Elvas, Planalto Horizonte, Bom Jesus, Piauí, 64900-000 Brazil

Contribution: Conceptualization, Data curation, Methodology, Validation, Visualization, Writing - review & editing

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Paula Rose de Almeida Ribeiro

Paula Rose de Almeida Ribeiro

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Contribution: Conceptualization, Formal analysis, Methodology, Visualization, Writing - review & editing

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Fernanda de Carvalho

Fernanda de Carvalho

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Contribution: Formal analysis, Methodology, Visualization, Writing - review & editing

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Márcia Rufini

Márcia Rufini

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Contribution: Conceptualization, Formal analysis, Methodology, Visualization, Writing - review & editing

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Aline Oliveira Silva

Aline Oliveira Silva

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Contribution: Conceptualization, ​Investigation, Methodology, Visualization, Writing - review & editing

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Anita Fernanda dos Santos Teixeira

Anita Fernanda dos Santos Teixeira

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Contribution: Conceptualization, Data curation, Methodology, Software, Validation, Writing - review & editing

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Thiago de Assis Pereira

Thiago de Assis Pereira

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Contribution: Conceptualization, Methodology, Visualization, Writing - review & editing

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Flávia Reis Sales

Flávia Reis Sales

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Contribution: Conceptualization, Methodology, Visualization, Writing - review & editing

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Fatima Maria de Souza Moreira

Corresponding Author

Fatima Maria de Souza Moreira

Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box 3037, Lavras, Minas Gerais, CEP 37200–900 Brazil

Correspondence

Fatima Maria de Souza Moreira, Setor de Biologia, Microbiologia e Processos Biológicos do Solo do Departamento de Ciência do Solo da Universidade Federal de Lavras, P.O. Box: 3037, CEP: 37200–900, Lavras, Minas Gerais - Brazil

Email: [email protected]

Contribution: Conceptualization, Data curation, Conceptualization, Data curation, ​Investigation, Methodology, ​Investigation, Methodology, Resources, Supervision, Writing - review & editing

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First published: 11 May 2021

Assigned to Associate Editor Lindsey Slaughter.

Abstract

Lima bean (Phaseolus lunatus L.) is the second most important species of the Phaseolus genus worldwide. In Brazil, it is grown primarily in the semiarid Northeast region. Although lima bean establishes symbiosis with N2-fixing bacteria (rhizobia), there is not yet a recommended inoculant. The aim of this study was to phenotypically and genotypically characterize rhizobia strains isolated from root nodules of lima bean collected in a Northeast region, and to evaluate their efficiency in supplying N nutrition to the crop under axenic conditions and in a Humic Rhodic Hapludox with and without correction of acidity. The symbiotic efficiencies were evaluated in a greenhouse under axenic conditions and, afterward, in pots with nonautoclaved soil with and without liming. Treatments consisted of the strains under evaluation, controls without inoculation (with low or high concentration or without mineral N), and a strain efficient for the crop. In all, 66 strains were isolated from the root nodules with predominance of strains belonging to the Bradyrhizobium genus. Liming brought about increases in lima bean development as it interacted with some strains. Six strains of the Bradyrhizobium genus, belonging to groups of type strains that include B. elkanii and B. japonicum, stood out for their biotechnological potential as inoculants for lima bean, and they should be tested in the field with the aim of validating their recommendation as inoculants for that crop.

CONFLICT OF INTEREST

Authors declare no conflicts of interest.