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Volume 29, Issue 3 cropsci1989.0011183X002900030035x p. 707-712
Crop Breeding, Genetics, & Cytology

Allelic Composition of Cotton at the Le1 and Le2 Loci.

William L. Rooney

William L. Rooney

Dep. of Soil and Crop Sciences, Texas A&M Univ., College Station, TX, 77843-2474

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David M. Stelly

Corresponding Author

David M. Stelly

Dep. of Soil and Crop Sciences, Texas A&M Univ., College Station, TX, 77843-2474

Corresponding author.Search for more papers by this author
First published: 01 May 1989
Citations: 1

Contribution from USDA-CRGO research grant 86-CRCR-1-2129 and the Texas A&M Univ. Undergraduate Fellows Program.

Abstract

Cotton (Gossypium spp.) zygotes bearing a Le2dav allelle in combination with Le1 and/or Le2 undergo a physiological reaction resulting in embryo or seedling in viability. All F1 hybrids between cultivated tetraploid cotton (G. hirsutum L. and G. barbadense L., 2n = 4x = 52) and a Le2dav homozygote are inviable. A breeding procedure being developed for mass extraction of doubled haploids of cotton relies on the “bio-elimination” of F1 hybrids by this complementary lethal system. Success is contingent on the absence of le1le2 gametes from a prospective parent because these alleles are fully compatible with Le2dav. The primary objective of this research was to determine the frequency of le1 and le2 in a representative sample of USA-developed G. hirsutum cultivars. A secondary objective was to determine if the timing of the incompatibility reaction was consistent across genetic backgrounds. Fifty-two cultivars were tested by observing the frequencies of nonviable vs. viable progeny and the timing of necrosis following two types of matings: (i) cultivar ✕ le1le1Le2davLe2dav and (ii) (cultivar ✕ le1le1le2le2) ✕ le1le1Le2davLe2dav. All 52 cultivars were found to be Le1Le1Le2Le2, indicating that the frequencies of alleles le1 and le2 are zero, or nearly so, in USA-developed G. hirsutum germplasm. Results on necrotic development indicated that the cumulative dosage of alleles Le1 and Le2 affected the onset of necrosis in the presence of Le2dav. Because alleles le1 and le2 are rare in American upland cottons, the doubled-haploid breeding system will be applicable to these stocks.